Coding

Part:BBa_K900002:Design

Designed by: Austin Jones   Group: iGEM12_Berkeley   (2012-10-02)

Nucleus Targeting Protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 97
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene was codon optimized for yeast and internal restriction sites were removed for compatibility with our Golden Gate cloning scheme.

Source

Isolated from the yeast genome through PCR.

References